Search results for "UNESCO::CIENCIAS DE LA VIDA::Microbiología"
showing 10 items of 62 documents
Evolutionary genomics and functional studies on the metabolic role of Blattabacterium, primary endosymbiont of cockroaches
2013
Introducció La simbiosi, entesa com la relació de interdependència entre dos o més individus de diferents espècies, ha tingut un profunda influència al llarg de l’evolució de la vida. De fet, hi ha un ampli consens respecte a la importància de les associacions simbiòtiques, en l’aparició i evolució primerenca de la cèl•lula eucariota, així com en l’evolució del sistema immunitari en eucariotes complexos o en l’adquisició de noves capacitats metabòliques en plantes i animals, permetent-los així explorar noves fonts d’aliments o ocupar nous nínxols ecològics. Segons l’efecte de la interacció sobre els membres de l’associació, es poden distingir tres tipus: comensalisme, quan un dels membres e…
Epidemiología molecular y genómica de aislados resistentes de Pseudomonas aeruginosa de origen hospitalario.
2019
Pseudomonas aeruginosa es un patógeno frecuente en entornos hospitalarios que acumula multitud de resistencias a antibióticos. Ante la dificultad de encontrar tratamiento efectivo, los pacientes están mucho tiempo colonizados y aumenta la probabilidad de transmisión. El principal objetivo del presente trabajo ha sido mostrar cómo el estudio evolutivo a nivel de genomas completos puede ser útil para la detección de transmisiones a diferentes niveles. Se decidió aplicar la misma metodología de reconstrucción de la filogenia a partir de las variantes encontradas en genomas completos que además se dataron por métodos bayesianos. Para ello, hemos contado con muestras de P. aeruginosa multirresis…
Autentificación de cepas de la CECT mediante MALDI-TOF MS y GC FAME
2013
Las colecciones de cultivo microbianas son organizaciones, generalmente oficiales, cuya principal misión es conservar a largo plazo cepas de microorganismos, y suministrarlas, previa petición, a laboratorios, empresas y centros que requieran microorganismos para investigación, controles de calidad, docencia o para numerosas aplicaciones biotecnológicas. Además, las colecciones de cultivo, para ser consideradas como tal, deben cumplir ciertas normas relacionadas con la recolección, la autentificación, el mantenimiento y la distribución de los cultivos de microorganismos, así como la catalogación y los sistemas de información Una de las tareas más importantes en una colección pública es que e…
Identification of Vibrio spp. (other than V. vulnificus) recovered on CPC agar from marine natural samples
2000
Two hundred and eighty four presumptive but not confirmed Vibrio vulnificus isolates grown on cellobiose-polymixin B-colistin agar (CPC) at 40°C, recovered from sea water samples from Valencia, Spain, during a microbiological survey for V. vulnificus, were phenotypically identified. Most of the isolates (91%) corresponded to Vibrio species. V. harveyi (24%) and V. splendidus(19%) were the most abundant species identified, followed by V. navarrensis (13%), V. alginolyticus (8%) and V. parahaemolyticus (5%). The ability to grow on CPC agar and ferment cellobiose of several V. vulnificus strains from different origins and serovars, including reference strains, was tested. Most serovar E isolat…
Evolution and comparative genomics of di-symbiotic systems in aphids from the Lachninae subfamily and genome reduction in Serratia symbiotica
2016
Ph.D. thesis presented by Alejandro Manzano Marín Director: Prof. Dr. Amparo Latorre Castillo Institution: Universiat de València
Bases moleculares de la señalización mediada por dUTPasas triméricas de bacteriófagos
2021
Tesis doctoral, 328 p. Figuras y tablas.
Comparative biochemistry of CO2 fixation and the evolution of autotrophy
1999
Carbon dioxide fixation is a polyphyletic trait that has evolved in widely separated prokaryotic branches. The three principal CO2-assimilation pathways are (i) the reductive pentose-phosphate cycle, i. e. the Calvin-Benson cycle; (ii) the reductive citric acid (or Arnon) cycle; and (iii) the net synthesis of acetyl-CoA from CO/CO2, or Wood pathway. Sequence analysis and the comparative biochemistry of these routes suggest that all of them were shaped to a considerable extent by the evolutionary recruitment of enzymes. Molecular phylogenetic trees show that the Calvin-Benson cycle was a relatively late development in the (eu)bacterial branch, suggesting that some form(s) of carbon assimilat…
Candida albicans fibrinogen binding mannoprotein: expression in clinical strains and immunogenicity in patients with candidiasis
1998
A 58 kDa cell wall-associated fibrinogen binding mannoprotein (mp58), previously characterized by our group in a Candida albicans laboratory strain (ATCC 26555), was found to be also present in the cell wall of clinical isolates of this fungus. Most strains examined appear to have functional mp58 species, as detected by their ability to bind fibrinogen. Western immunoblot analysis, with a monovalent polyclonal antibody generated against the mp58 species from strain ATCC 26555, revealed differences in recognition patterns depending on the strain tested and the culture conditions used. Serum samples from normal and Candida infected individuals were examined for the presence of antibodies agai…
The cell wall structure: developments in diagnosis and treatment of candidiasis
1998
Candidiasis are among the fungal infections the most difficult to diagnose and treat. Research focused on specific fungal components which are absent in the host, such as the cell wall has lead to a better understanding of Candida albicans pathogenicity and clinical impact. The cell wall is responsible for antigenic expression and primary interaction with the host. It is composed mainly of b-glucans, chitin and mannoproteins, which account for the rigidity of the wall and for the fungal morphology. Of these components, mannoproteins might carry a “morphogenetic code” which might modulate the molecular architecture of the cell wall. The features of specific cell wall proteins as part of buil…
Saccharomyces cerevisiae Rds2 transcription factor involvement in cell wall composition and architecture
2008
Although the cell wall is very important in yeasts, relatively little is known about the relationship between its structure and function. In Saccharomyces cerevisiae, a family of 55 transcription factor proteins unique to fungi, so-called zinc cluster proteins, has been described. Of these, Rds2 has been identified as an activator/inhibitor of gluconeogenesis. However, previous studies have pointed out additional roles for this protein, specifically, in the modulation of cell-wall architecture and drug sensitivity. In this work, evidence regarding the role of Rds2 as a regulator of cell-wall architecture and composition is presented based on phenotypical analysis of the cell walls prepared …